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Facile fluorescence strategy for sensitive detection of microcystin-LR based on dsDNA-templated copper nanoclusters

journal contribution
posted on 2020-01-01, 00:00 authored by Y Zhang, Y Lai, X Teng, S Pu, Z Yang, P Pang, H Wang, C Yang, Wenrong YangWenrong Yang, Colin BarrowColin Barrow
We developed a simple, sensitive, low-cost and label-free method to detect microcystin-leucine-arginine (MC-LR) using double-strand DNAlated copper nanoclusters (dsDNA-CuNCs) as fluorescent probes. The dsDNA template was designed and fabricated by a MC-LR aptamer strand and its complementary strand, which facilitated CuNCs formation and recognized target MC-LR specifically with strong affinity. The formed fluorescent sensing probe of dsDNA-CuNCs exhibited maximum emission wavelength at 575 nm. Upon addition of target MC-LR into dsDNA-CuNCs, fluorescence was quenched considerably due to the high affinity between MC-LR and the aptamer strand, which indicated a conformational change of the dsDNA-CuNCs probe from the dsDNA structure to single-stranded DNA. Then, the change in fluorescence intensity was used to monitor the MC-LR concentration from 0.01 to 1000 μg L-1 with a limit of detection of 4.8 ng L-1. Compared with previous reports, this method did not require design of a complex DNA sequence, fluorescence dye labels or sophisticated experimental techniques. Moreover, the proposed sensing system could be applicable for detection of target MC-LR in real-water samples.

History

Journal

Analytical Methods

Volume

12

Issue

13

Pagination

1752 - 1758

Publisher

Royal Society of Chemistry

Location

London, Eng.

ISSN

1759-9660

eISSN

1759-9679

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2020, The Royal Society of Chemistry