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Fast ion chromatography using short anion exchange columns
Version 2 2024-06-05, 00:52Version 2 2024-06-05, 00:52
Version 1 2019-07-11, 09:30Version 1 2019-07-11, 09:30
journal contribution
posted on 2024-06-05, 00:52 authored by E Tyrrell, Robert ShellieRobert Shellie, EF Hilder, CA Pohl, PR HaddadA fast ion chromatographic system is described which uses shorter column lengths and compares various eluent profiles in order to maximise the performance without sacrificing the chromatographic resolution. Both isocratic and gradient elution profiles were considered to find the most efficient mode of separation. The separation and determination of seven target anions (chloride, chlorate, nitrate, chromate, sulfate, thiocyanate and perchlorate) was achieved using a short (4 mm ID, 50 mm long) column packed with Dionex AS20 high-capacity anion exchange material. A hydroxide eluent was used at an initial concentration of 25 mM (at a flow-rate of 1.0 mL/min) and two performance maxima were found. The maximum efficiency occurred at a normalised gradient ramp rate of 5 mM/t0, resulting in a peak capacity of 16, while the fastest separation (<3 min) occurred at a normalised ramp rate of 30 mM/t0. The retention time, peak width and resolution using the different eluent profiles on varying column lengths is also compared. Further investigations in this study determined that the highest peak capacity separation under gradient conditions could be approximated using an isocratic separation. The advantage of using this novel approach to approximate the maximum efficiency separation removes the need for column re-equilibration that is required for gradient elution resulting in faster analyses and enhanced sample throughput, with benefits in particular for multidimensional chromatography. © 2009 Elsevier B.V. All rights reserved.
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Journal
Journal of Chromatography AVolume
1216Pagination
8512-8517Location
Amsterdam, The NetherlandsPublisher DOI
ISSN
0021-9673Language
engPublication classification
C1.1 Refereed article in a scholarly journalIssue
48Publisher
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