mak-gogpolsupplied-2001.pdf (507.94 kB)
Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1
journal contributionposted on 2001-08-01, 00:00 authored by M Hill, C Hooker, D Harrich, S Crowe, Johnson Mak
The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is generated by ribosomal frameshifting. The multimerization of Gag and Gag-Pol is an essential step in the formation of infectious viral particles. In this study, we examined whether the interaction between Gag and Gag-Pol is initiated during protein translation in order to facilitate the trafficking and subsequent packaging of Gag-Pol into the virion. A conditional cotransfection system was developed in which virion formation required the coexpression of two HIV-1-based plasmids, one that produces both Gag and Gag-Pol and one that only produces Gag-Pol. The Gag-Pol proteins were either immunotagged with a His epitope or functionally tagged with a mutation (K65R) in reverse transcriptase that is associated with drug resistance. Gag-Pol packaging was assessed to determine whether the Gag-Pol incorporated into the virion was preferentially packaged from the plasmid that expressed both Gag and Gag-Pol or whether it could be packaged from either plasmid. Our data show that translation of Gag and Gag-Pol from the same mRNA is not critical for virion packaging of the Gag-Pol polyprotein or for viral function.
JournalJournal of virology
Pagination6835 - 6840
PublisherAmerican Society for Microbiology
LocationWashington, D. C.
Publication classificationC1.1 Refereed article in a scholarly journal
Copyright notice2001, American Society for Microbiology
CategoriesNo categories selected
fusion proteinsgag-polgene productsgagHIV-1protein biosynthesisRNAmessengerviral RNAgenetic transcriptiontransfectionvirus assemblyScience & TechnologyLife Sciences & BiomedicineVirologyASSEMBLY INTERMEDIATE COMPLEXESIN-VITROREVERSE-TRANSCRIPTASEPRECURSOR PROTEINWILD-TYPEPARTICLESREGIONPOLYPROTEINVIRIONSCELLS