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Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1

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journal contribution
posted on 2001-08-01, 00:00 authored by M Hill, C Hooker, D Harrich, S Crowe, Johnson Mak
The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is generated by ribosomal frameshifting. The multimerization of Gag and Gag-Pol is an essential step in the formation of infectious viral particles. In this study, we examined whether the interaction between Gag and Gag-Pol is initiated during protein translation in order to facilitate the trafficking and subsequent packaging of Gag-Pol into the virion. A conditional cotransfection system was developed in which virion formation required the coexpression of two HIV-1-based plasmids, one that produces both Gag and Gag-Pol and one that only produces Gag-Pol. The Gag-Pol proteins were either immunotagged with a His epitope or functionally tagged with a mutation (K65R) in reverse transcriptase that is associated with drug resistance. Gag-Pol packaging was assessed to determine whether the Gag-Pol incorporated into the virion was preferentially packaged from the plasmid that expressed both Gag and Gag-Pol or whether it could be packaged from either plasmid. Our data show that translation of Gag and Gag-Pol from the same mRNA is not critical for virion packaging of the Gag-Pol polyprotein or for viral function.

History

Journal

Journal of virology

Volume

75

Issue

15

Pagination

6835 - 6840

Publisher

American Society for Microbiology

Location

Washington, D. C.

ISSN

0022-538X

eISSN

1098-5514

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2001, American Society for Microbiology