Deakin University
Browse
t040701-Singh2017-Article-GeneSpecificIm.pdf (829.81 kB)

Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease

Download (829.81 kB)
journal contribution
posted on 2023-10-24, 05:08 authored by S Singh, A Kaushal, S Gupta, A Kumar
© 2016, Association of Microbiologists of India. An impedimetric mga gene specific DNA sensor was developed by immobilization of single stranded DNA probe onto the screen printed modified gold-dendrimer nanohybrid composite electrode for early and rapid detection of S. pyogenes in human throat swab samples causing rheumatic heart disease. Electrochemical impedance response was measured after hybridization with bacterial single stranded genomic DNA (ssG-DNA) with probe. The sensor was found highly specific to S. pyogenes and can detect as low as 0.01 ng ssDNA in 6 µL sample only in 30 min. The nanohybrid sensor was also tested with non-specific pathogens and characterized by FTIR. An early detection of the pathogen S. pyogenes in human can save damage of mitral and aortic heart valves (rheumatic heart disease) by proper medical care.

History

Journal

Indian Journal of Microbiology

Volume

57

Pagination

112-115

Open access

  • Yes

ISSN

0046-8991

Publication classification

C2.1 Other contribution to refereed journal

Issue

1

Publisher

Springer

Usage metrics

    Research Publications

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC