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Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes.
journal contributionposted on 2005-07-19, 00:00 authored by Anthony Civitarese, M Hesselink, Aaron RussellAaron Russell, E Ravussin, P Schrauwen
Ingestion of carbohydrate during exercise may blunt the stimulation of fat oxidative pathways by raising plasma insulin and glucose concentrations and lowering plasma free fatty acid (FFA) levels, thereby causing a marked shift in substrate oxidation. We investigated the effects of a single 2-h bout of moderate-intensity exercise on the expression of key genes involved in fat and carbohydrate metabolism with or without glucose ingestion in seven healthy untrained men (22.7 ± 0.6 yr; body mass index: 23.8 ± 1.0 kg/m2; maximal O2 consumption: 3.85 ± 0.21 l/min). Plasma FFA concentration increased during exercise (P < 0.01) in the fasted state but remained unchanged after glucose ingestion, whereas fat oxidation (indirect calorimetry) was higher in the fasted state vs. glucose feeding (P < 0.05). Except for a significant decrease in the expression of pyruvate dehydrogenase kinase-4 (P < 0.05), glucose ingestion during exercise produced minimal effects on the expression of genes involved in carbohydrate utilization. However, glucose ingestion resulted in a decrease in the expression of genes involved in fatty acid transport and oxidation (CD36, carnitine palmitoyltransferase-1, uncoupling protein 3, and 5'-AMP-activated protein kinase-α2; P < 0.05). In conclusion, glucose ingestion during exercise decreases the expression of genes involved in lipid metabolism rather than increasing genes involved in carbohydrate metabolism.
JournalAmerican journal of physiology: endocrinology and metabolism
Pagination1023 - 1029
PublisherAmerican Physiological Society
Publication classificationC1.1 Refereed article in a scholarly journal; C Journal article
Copyright notice2005, American Physiological Society
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skeletal muscle gene expressionexercise-diet interactionScience & TechnologyLife Sciences & BiomedicineEndocrinology & MetabolismPhysiologyACTIVATED PROTEIN-KINASESHORT-TERM EXERCISEMETABOLIC GENESTRANSCRIPTIONAL ACTIVATIONCARBOHYDRATE INGESTIONPROLONGED EXERCISEUCP3 EXPRESSIONRECEPTOR-ALPHAACID OXIDATIONGLYCEMIC INDEX