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Gold-Nanorod-Assisted Near-Infrared Stimulation of Primary Auditory Neurons

journal contribution
posted on 2024-08-22, 05:43 authored by J Yong, K Needham, WGA Brown, BA Nayagam, Sally McArthurSally McArthur, A Yu, PR Stoddart
Infrared stimulation offers an alternative to electrical stimulation of neuronal tissue, with potential for direct, non‐contact activation at high spatial resolution. Conventional methods of infrared neural stimulation (INS) rely on transient heating due to the absorption of relatively intense laser beams by water in the tissue. However, the water absorption also limits the depth of penetration of light in tissue. Therefore, the use of a near‐infrared laser at 780 nm to stimulate cultured rat primary auditory neurons that are incubated with silica‐coated gold nanorods (Au NRs) as an extrinsic absorber is investigated. The laser‐induced electrical behavior of the neurons is observed using whole‐cell patch clamp electrophysiology. The nanorod‐treated auditory neurons (NR‐ANs) show a significant increase in electrical activity compared with neurons that are incubated with non‐absorbing silica‐coated gold nanospheres and control neurons with no gold nanoparticles. The laser‐induced heating by the nanorods is confirmed by measuring the transient temperature increase near the surface of the NR‐ANs with an open pipette electrode. These findings demonstrate the potential to improve the efficiency and increase the penetration depth of INS by labeling nerves with Au NRs and then exposing them to infrared wavelengths in the water window of tissue.

History

Journal

Advanced Healthcare Materials

Volume

3

Pagination

1862-1868

Location

Germany

ISSN

2192-2640

eISSN

2192-2659

Language

English

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

11

Publisher

WILEY