Deakin University
Browse
kong-goldnanoparticlesmodulate-2019.pdf (5.54 MB)

Gold nanoparticles modulate the crosstalk between macrophages and periodontal ligament cells for periodontitis treatment

Download (5.54 MB)
Version 2 2024-06-06, 09:16
Version 1 2019-04-11, 15:54
journal contribution
posted on 2024-06-06, 09:16 authored by C Ni, J Zhou, N Kong, T Bian, Y Zhang, X Huang, Y Xiao, Wenrong YangWenrong Yang, F Yan
The regeneration of lost periodontal apparatus in periodontitis treatment remains a clinical challenge due to the limited regenerative capacity of cementum, periodontal ligament and alveolar bone in periodontitis condition. For periodontal tissue regeneration, it is essential to regulate the inflammatory response and the subsequent differentiation of periodontal cells under the condition due to the infectious nature of the disease. In this study, it was noted that 45 nm gold nanoparticles (AuNPs) could exhibit significant anti-inflammatory effect and improve the periodontal inflammatory microenvironment via regulating inflammatory and regenerative cytokine production and modulating macrophage polarization, subsequently affect the differentiation of human periodontal ligament cells (hPDLCs). With the addition of direct effects of AuNPs on hPDLCs, the periodontal tissue differentiation capacity of hPDLCs in LPS-activated inflammatory macrophage-hPDLCs coculture system was significantly enhanced by the interaction between AuNPs-conditioned macrophage and AuNPs-stimulated hPDLCs. The potential therapeutic application of AuNPs in periodontal tissue regeneration and periodontitis treatment was investigated using both rat fenestration and ligature-induced periodontitis models. It was found that the treatment of 45 AuNPs showed significantly increased newly-formed periodontal attachment, bone and cementum in periodontal defect and less tissue destruction in the progression of periodontitis. This study demonstrated that 45 nm AuNPs could not only directly modulate hPDLCs, but also regulate the early inflammatory response of periodontal tissues via the regulation of macrophage phenotypes, therefore, generate a microenvironment with constraint inflammatory cytokine levels and reparative cytokines such as bone morphogenetic protein-2 (BMP-2), leading to PDLC differentiation, periodontal tissue regeneration and the prevention of periodontitis progression.

History

Journal

Biomaterials

Volume

206

Pagination

115-132

Location

Netherlands

Open access

  • Yes

ISSN

0142-9612

eISSN

1878-5905

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2019, The Authors

Publisher

ELSEVIER SCI LTD