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Graphene-enhanced 3D chemical mapping of biological specimens at near-atomic resolution
journal contribution
posted on 2018-08-08, 00:00 authored by V R Adineh, C Zheng, Q Zhang, Ross MarceauRoss Marceau, B Liu, Y Chen, K J Si, M Weyland, T Velkov, W Cheng, J Li, J Fu© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim The direct imaging of individual atoms within the cellular context holds great potential for understanding the fundamental physical and chemical processes in organisms. Here, a novel approach for imaging of electrically insulated biological cells by introducing a graphene encapsulation approach to “disguise” the low-conductivity barrier is reported. Upon successful coating using a water-membrane-based protocol, the electrical properties of the graphene enable voltage pulsing field evaporation for atom probe tomography (APT). Low conductive specimens prepared from both Au nanoparticles and antibiotic-resistant bacterial cells have been tested. For the first time, a significant graphene-enhanced APT mass resolving power is also observed confirming the improved compositional accuracy of the 3D data. The introduction of 2D materials encapsulation lays the foundation for a breakthrough direction in specimen preparation from nanomembrane and nanoscale biological architectures for subsequent 3D near-atomic characterization.
History
Journal
Advanced functional materialsVolume
28Issue
32Article number
1801439Publisher
WileyLocation
Chichester, Eng.Publisher DOI
Link to full text
ISSN
1616-301XeISSN
1616-3028Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, WILEY-VCH VerlagUsage metrics
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No categories selectedKeywords
Science & TechnologyPhysical SciencesTechnologyChemistry, MultidisciplinaryChemistry, PhysicalNanoscience & NanotechnologyMaterials Science, MultidisciplinaryPhysics, AppliedPhysics, Condensed MatterChemistryScience & Technology - Other TopicsMaterials SciencePhysicsatom probe tomographycellular imaginggrapheneION MASS-SPECTROMETRYPROBE TOMOGRAPHYCRYOELECTRON MICROSCOPYMULTILAYER GRAPHENEPLASMENE NANOSHEETSFIELDPROSPECTSGRAPHITECELLSTIPS
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