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Graphene nanodots encaged 3-D gold substrate as enzyme loading platform for the fabrication of high performance biosensors
journal contribution
posted on 2015-12-01, 00:00 authored by Jianmei Wang, H Zhu, Y Xu, Wenrong YangWenrong Yang, A Liu, F Shan, M Cao, J LiuHerein, a uniform three-dimensional (3-D) graphene nanodots-encaged porous gold electrode was prepared via ion beam sputtering deposition (IBSD) and mild corrosion chemistry for efficient enzyme electrode fabrication. Enzymes, like glucose oxidase and catalase, were modified with pyrene functionalities and then loaded into the graphene nanodots encaged porous gold electrode via non-covalent π-π stacking interaction between pyrene and graphene. The fabricated enzyme electrodes showed profound reusability and repeatability, high sensitivity, inherent selectivity and enhanced detection range. As for glucose analysis a broad linear range from 0.05 to 100 mM was obtained and the linear range for hydrogen peroxide was 0.005 to 4 mM. Detection limits of 30 μM for glucose and 1 μM for hydrogen peroxide were achieved (S/N = 3), respectively. These electrodes can be applied to analyze the clinical samples with reliable results. The formation mechanism and 3-D structure of the porous electrode were investigated using high resolution transmission electron microscope (HRTEM), atomic force microscopy (AFM), scanning electron microscope (SEM) and electrochemical impedance spectroscopy (EIS). Most importantly, various other ideal biosensors can be fabricated using the same porous electrode and the same enzyme modification methodology.
History
Journal
Sensors and actuators, b: chemicalVolume
220Pagination
1186 - 1195Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0925-4005Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, ElsevierUsage metrics
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No categories selectedKeywords
Science & TechnologyPhysical SciencesTechnologyChemistry, AnalyticalElectrochemistryInstruments & InstrumentationChemistryBiosensorsGraphene nanodotsPorous goldIon beam sputtering depositionGlucose oxidaseCatalaseGLUCOSE-OXIDASEELECTROCHEMICAL BIOSENSORSNANOTUBE ELECTRODESHYDROGEN-PEROXIDESENSORSFILMS
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