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Guanylyl cyclase stimulation by homologous and heterologous natriuretic peptides in salmonids

journal contribution
posted on 2002-01-01, 00:00 authored by Will Callahan, H McWilliam, M Powell, Marie-Therese Toop
A range of homologous (trout ANP, trout CNP, trout VNP) and heterologous (eel ANP, eel ANP-NH2, rat ANP, porcine CNP) NPs were tested for their effect on guanylyl cyclase in gill and kidney membrane preparations from freshwater and seawater-acclimated rainbow trout and Atlantic salmon. All NPs stimulated guanylyl cyclase at 1 µmol l-1in all preparations. ANP was the most potent stimulator of kidney guanylyl cyclase and CNP the most potent stimulator of guanylyl cyclase in gills. Some differences were apparent between the potencies of homologous and heterologous peptides at 1 µmol l-1: tANP was more potent than rANP in the SW trout kidney and tCNP was more potent than pCNP in FW salmon tissues. While eANP was more potent than tANP in trout gills, it was less potent than tANP in FW salmon gills. However, there was no significant difference between the potencies of eANP and eANP-NH2 in trout or salmon gills. Salinity did not affect guanylyl cyclase activity with the exception that trout ANP at 1 µmol l-1was more potent in SW trout kidneys than in FW trout kidneys. These results suggest a predomination of NPR-A in the kidney and NPR-B in the gill. It appears that salmonid NPR-A and NPR-B are relatively promiscuous in their ligand affinity, with few differences in the potencies of trout and mammalian NPs and only small differences in cGMP production where these differences do occur.

History

Journal

Fish physiology and biochemistry

Volume

26

Issue

2

Pagination

129 - 138

Publisher

Kluwer Academic Publishers

Location

Amsterdam, Netherlands

ISSN

0920-1742

eISSN

1573-5168

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2003, Kluwer Academic Publishers