Halophilic archaea in the human intestinal mucosa
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journal contribution
posted on 2024-06-05, 04:08 authored by Andrew OxleyAndrew Oxley, MP Lanfranconi, D Würdemann, S Ott, S Schreiber, TJ McGenity, KN Timmis, B NogalesThe human gastrointestinal tract microbiota, despite its key roles in health and disease, remains a diverse, variable and poorly understood entity. Current surveys reveal a multitude of undefined bacterial taxa and a low diversity of methanogenic archaea. In an analysis of the microbiota in colonic mucosal biopsies from patients with inflammatory bowel disease we found 16S rDNA sequences representing a phylogenetically rich diversity of halophilic archaea from the Halobacteriaceae (haloarchaea), including novel phylotypes. As the human colon is not considered a salty environment and haloarchaea are described as extreme halophiles, we evaluated and further discarded the possibility that these sequences originated from pre-colonoscopy saline lavage solutions. Furthermore, aerobic enrichment cultures prepared from a patient biopsy at low salinity (2.5% NaCl) yielded haloarchaeal sequence types. Microscopic observation after fluorescence in situ hybridization provided evidence of the presence of viable archaeal cells in these cultures. These results prove the survival of haloarchaea in the digestive system and suggest that they may be members of the mucosal microbiota, even if present in low numbers in comparison with methanogenic archaea. Investigation of a potential physiological basis of this association may lead to new insights into gastrointestinal health and disease. © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
History
Journal
Environmental microbiologyVolume
12Pagination
2398-2410Location
Chichester, Eng.ISSN
1462-2912eISSN
1462-2920Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2010, Society for Applied Microbiology and Blackwell Publishing Ltd.Issue
9Publisher
WileyUsage metrics
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