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High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples
journal contributionposted on 2010-08-18, 00:00 authored by Geoffrey McDermott, L Noonan, M Mnatsakanyan, R Shalliker, Xavier ConlanXavier Conlan, Neil BarnettNeil Barnett, Paul FrancisPaul Francis
An improved post-column 2,2´-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH• response were studied in a univariate approach. Optimum conditions were found to be: 5×10−5M DPPH• reagent prepared in a 75% methanol: 25% 40mM citric acid–sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2m×0.25mm i.d. PEEK tubing; detection at 521 nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies.
JournalAnalytica chimica acta
Pagination76 - 82
LocationAmsterdam, The Netherlands
NotesAvailable online 23 July 2010
Publication classificationC1 Refereed article in a scholarly journal
Copyright notice2010, Elsevier B.V.
CategoriesNo categories selected
2´-Diphenyl-1-picrylhydrazylradical scavenging assayantioxidantshigh-performance liquid chromatographygreen teathymeScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistry2,2 '-Diphenyl-1-picrylhydrazylDPPH SCREENING METHODANTIOXIDANT ACTIVITYWATER EXTRACTSRAPID IDENTIFICATIONONLINE DETECTIONACACIA-CONFUSAHPLCPHYTOCHEMICALSCOMPONENTSMIXTURES