High temperature liquid chromatography with monolithic capillary columns and pure water eluent

Use of an organic polymeric monolithic stationary phase for high temperature liquid chromatography (HTLC) with a pure water eluent is demonstrated for the first time. Separations of n-alcohols at temperatures exceeding 200°C were achieved with no statistically significant reduction in chromatographic performance evident after continuous operation for 1000 column volumes above 200°C.