Deakin University
Browse

File(s) not publicly available

Hormone-Dependent Expression of the Ovine B-Lactoglobulin Gene

Version 2 2024-06-13, 14:57
Version 1 2021-11-22, 10:29
journal contribution
posted on 2024-06-13, 14:57 authored by R Osborne, M Howell, AJ Clark, KR Nicholas
SUMMARYThe minimal hormonal requirements for inducing the ovine β-lactoglobulin gene have been investigated using mammary gland explants from ewes in the first half of pregnancy. Quantification of β-lactoglobulin mRNA showed that a combination of insulin, Cortisol and prolactin was required to stimulate the expression of the gene and that this response could not be enhanced by the addition of oestrogen and thyroid hormone to the culture medium. Explants cultured in the presence of insulin, Cortisol and prolactin also demonstrated the capacity to synthesize the protein. Progesterone did not inhibit the induction of the gene, which is consistent with the increase in β-lactoglobulin mRNA observed in vivo in the mammary gland during the final 2 months of pregnancy when the circulating level of progesterone is elevated.

History

Journal

Journal of Dairy Research

Volume

62

Pagination

321-329

Location

England

ISSN

0022-0299

eISSN

1469-7629

Language

en

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

2

Publisher

Cambridge University Press (CUP)