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IEF pattern classification-derived criteria for the identification of epoetin-delta in urine
journal contribution
posted on 2010-06-01, 00:00 authored by Severine LamonSeverine Lamon, J Boccard, P E Sottas, N Glatz, G Wuerzner, N Robinson, M SaugyEpoetin-δ (Dynepo™ Shire Pharmaceuticals, Basing stoke, UK) is a synthetic form of erythropoietin (EPO) whose resemblance with endogenous EPO makes it hard to identify using the classical identification criteria. Urine samples collected from six healthy volunteers treated with epoetin-δ injections and from a control population were immuno-purified and analyzed with the usual IEF method. On the basis of the EPO profiles integration, a linear multivariate model was computed for discriminant analysis. For each sample, a pattern classification algorithm returned a bands distribution and intensity score (bands intensity score) saying how representative this sample is of one of the two classes, positive or negative. Effort profiles were also integrated in the model. The method yielded a good sensitivity versus specificity relation and was used to determine the detection window of the molecule following multiple injections. The bands intensity score, which can be generalized to epoetin-α and epoetin-β, is proposed as an alternative criterion and a supplementary evidence for the identification of EPO abuse.
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Journal
ElectrophoresisVolume
31Issue
12Pagination
1918 - 1924Publisher
Wiley - V C H Verlag GmbH & Co. KGaALocation
Weinheim, GermanyPublisher DOI
ISSN
0173-0835eISSN
1522-2683Language
engPublication classification
C1.1 Refereed article in a scholarly journalUsage metrics
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