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Identification of a hypervariable region in the long terminal repeat of equine infectious anemia virus

journal contribution
posted on 1991-03-01, 00:00 authored by S Carpenter, Soren AlexandersenSoren Alexandersen, M J Long, S Perryman, B Chesebro
An avirulent, field-derived isolate of equine infectious anemia virus (EIAV), designated MA-1, was molecularly cloned, and the complete nucleotide sequence was determined for the 3' half of the viral genome. Comparisons between MA-1 and the prototype Wyoming strain of EIAV identified a 66-nucleotide stretch between CAAT (-91) and TATAA (-25) in the U3 region of the long terminal repeat, where sequence divergence was as high as 39.3%. The polymerase chain reaction was used to amplify and clone long terminal repeat sequences from Th-1, the in vivo parental stock of MA-1. Results indicated that the nucleotide sequences of MA-1 and Th-1 clones were less variable than was observed between MA-1 and Wyoming. However, MA-1 and Th-1 markedly differed in the types of enhancer sequences located in the hypervariable region. These results suggest that variation in lentivirus regulatory sequences may be important in EIAV host cell tropism and pathogenesis.

History

Journal

Journal of virology

Volume

65

Issue

3

Pagination

1605 - 1610

Publisher

American Society for Microbiology

Location

Washington, D.C.

ISSN

0022-538X

eISSN

1098-5514

Language

eng

Publication classification

CN.1 Other journal article

Copyright notice

1991, American Society for Microbiology