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Identification of an integral plasma membrane pool of protein kinase C in mammalian tissues and cells
journal contribution
posted on 2005-09-01, 00:00 authored by Y Zhu, W Lim, B Tan, T Teo, Wei DuanWei DuanProtein kinase C (PKC) is a family of serine/threonine protein kinases that are pivotal in cellular regulation. Since its discovery in 1977, PKCs have been known as cytosolic and peripheral membrane proteins. However, there are reports that PKC can insert into phospholipids vesicles in vitro. Given the intimate relationship between the plasma membrane and the activation of PKC, it is important to determine whether such “membrane-inserted” form of PKC exists in mammalian cells or tissues. Here, we report the identification of an integral plasma membrane pool for all the 10 PKC isozymes in vivo by their ability to partition into the detergent-rich phase in Triton X-114 phase partitioning, and by their resistance to extractions with 0.2 M sodium carbonate (pH 11.5), 2 M urea and 2 M sodium chloride. The endogenous integral membrane pool of PKC in mouse fibroblasts is found to be acutely regulated by phorbol ester or diacylglycerol, suggesting that this pool of PKC may participate in cellular processes known to be regulated by PKC. At least for PKCα, the C2–V3 region at the regulatory domain of the kinase is responsible for membrane integration. Further exploration of the function of this novel integral plasma membrane pool of PKC will not only shed new light on molecular mechanisms underlying its cellular functions but also provide new strategies for pharmaceutical modulation of this important group of kinases.
History
Journal
Cellular signallingVolume
17Issue
9Pagination
1125 - 1136Publisher
Elsvier Inc.Location
New York, N.Y.ISSN
0898-6568eISSN
1873-3913Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2004, Elsevier Inc.Usage metrics
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