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Identification of expressed HSP`s in blacklip abalone (Haliotis rubra Leach) during heat and salinity stresses

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posted on 2001-12-01, 00:00 authored by B Drew, D Miller, Marie-Therese Toop, Peter Hanna
Both prokaryotes and eukaryotes express a set of highly conserved proteins in response to external and internal stress. The stressors include tissue trauma,anoxia, heavy metal toxicity, infection, changed salinity, and the mmost characterized, heat shock. The result is an expression of stress proteins or heat shock proteins (HSP's) which lead to protection of protein integrity, and also to tolerance under continued heat stress conditions. The Australian backflip abalone (Haliotis rubra) is found principally in southern coastal water and also in estuarine/bay environments. Esturaine/bay environments have greater fluctuations in environmental conditions, especially those of salinity and water temperature, than they are found along oceanic coasts. Abalone from esturaine/bay and oceanic coastal environments were subjected to either increased temperature (2° C/day for a total of 10°C) or hyposalinity (80% seawater). Esturaine/bay abolone were less affectes than the oceanic animals by temperature increase and also demonstrated the ability to volume regualte 3 h after the initial salinity shock. SDS-PAGE and Western blotting techniques, together with dot blots of total protein, using HSP70 specific antibodies, were used to detect HSP70s in the foot muscle of the animals and indicated an expression of HSP70 in response to heat shock in abalone, but not following hyposalinity shock. RT-PCR yeilded a partial cDNA clone of HSP70 from the foot muscle.

History

Journal

Journal of shellfish research

Volume

20

Issue

2

Pagination

695 - 703

Publisher

National Shellfisheries Association

Location

Baltimore, Md.

ISSN

0730-8000

eISSN

1943-6319

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2001, National Shellfisheries Association

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