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Immunostimulatory principles from Chlorella pyrenoidosa – part 1 : isolation and biological assessment in vitro

journal contribution
posted on 2007-01-10, 00:00 authored by J Kralovec, K Metera, J Kumar, L Watson, G Girouard, Y Guan, R Carr, Colin BarrowColin Barrow, H Ewart
Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin™), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin™ is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin™ is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose.

History

Journal

Phytomedicine

Volume

14

Issue

1

Pagination

57 - 64

Publisher

G. Fischer

Location

Stuttgart, Germany

ISSN

0944-7113

eISSN

1618-095X

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2005, Elsevier