Immunostimulatory principles from Chlorella pyrenoidosa – part 1 : isolation and biological assessment in vitro
journal contribution
posted on 2007-01-10, 00:00authored byJ Kralovec, K Metera, J Kumar, L Watson, G Girouard, Y Guan, R Carr, Colin BarrowColin Barrow, H Ewart
Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin™), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin™ is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin™ is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose.