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Impact of in vitro embryo culture and transfer on blood pressure regulation in the adolescent lamb

Version 2 2024-06-06, 03:05
Version 1 2020-11-19, 07:52
journal contribution
posted on 2024-06-06, 03:05 authored by M Padhee, IC McMillen, S Zhang, SM Maclaughlin, James ArmitageJames Armitage, GA Head, JRT Darby, JM Kelly, SR Rudiger, DO Kleemann, SK Walker, JL Morrison
AbstractNutrition during the periconceptional period influences postnatal cardiovascular health. We determined whether in vitro embryo culture and transfer, which are manipulations of the nutritional environment during the periconceptional period, dysregulate postnatal blood pressure and blood pressure regulatory mechanisms. Embryos were either transferred to an intermediate recipient ewe (ET) or cultured in vitro in the absence (IVC) or presence of human serum (IVCHS) and a methyl donor (IVCHS+M) for 6 days. Basal blood pressure was recorded at 19–20 weeks after birth. Mean arterial pressure (MAP) and heart rate (HR) were measured before and after varying doses of phenylephrine (PE). mRNA expression of signaling molecules involved in blood pressure regulation was measured in the renal artery. Basal MAP did not differ between groups. Baroreflex sensitivity, set point, and upper plateau were also maintained in all groups after PE stimulation. Adrenergic receptors alpha-1A (αAR1A), alpha-1B (αAR1B), and angiotensin II receptor type 1 (AT1R) mRNA expression were not different from controls in the renal artery. These results suggest there is no programmed effect of ET or IVC on basal blood pressure or the baroreflex control mechanisms in adolescence, but future studies are required to determine the impact of ET and IVC on these mechanisms later in the life course when developmental programming effects may be unmasked by age.

History

Journal

Journal of Developmental Origins of Health and Disease

Volume

12

Article number

PII S2040174420001014

Pagination

731-737

Location

England

ISSN

2040-1744

eISSN

2040-1752

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Issue

5

Publisher

CAMBRIDGE UNIV PRESS