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Improvement in non-programmable sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata

journal contribution
posted on 2023-11-06, 01:01 authored by Y Liu, X Li, T Xu, N Robinson, J Qin
This study assessed the effects of the addition of vitamins (. l-ascorbic acid), amino acids (glycine and taurine), and monosaccharides (glucose, fructose and galactose) on sperm cryopreservation using a non-programmable freezing technique in farmed greenlip abalone. The results showed that the addition of taurine, glycine or l-ascorbic acid significantly improved the post-thaw sperm motility, whereas the post-thaw sperm fertilization rates were improved by the addition of glycine or l-ascorbic acid. Flow cytometry analysis demonstrated that the addition of glycine significantly enhanced the post-thaw sperm plasma membrane integrity and acrosome integrity. Results from the investigation on monosaccharides demonstrated that glucose, fructose and galactose had a similar cryoprotective effect, resulting in a similar level of the post-thaw sperm fertilization rate, plasma membrane integrity, mitochondrial membrane potential and acrosome integrity. In this study, the highest post-thaw fertilization rate of 96% was achieved by using the cryoprotective mediums containing 6% DMSO, 1% glucose and 0.6% glycine.

History

Journal

Aquaculture

Volume

434

Pagination

362-366

ISSN

0044-8486

eISSN

1873-5622

Language

English

Publisher

ELSEVIER SCIENCE BV