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In situ imaging miRNAs using multifunctional linear DNA nanostructure
journal contributionposted on 2023-02-10, 04:00 authored by H Xu, M Lin, Y Zheng, X Fang, X Huang, Q Huang, J Xu, Wei DuanWei Duan, J Wei, L Jia
The microRNAs (miRNAs) play a critical role in many biological processes and are essential biomarkers for diagnosing disease. However, the sensitive and specific quantification of microRNAs (miRNAs) expression in living cells still faces a huge challenge. Our study designed a multifunctional linear DNA nanostructure (MLN) as a carrier of molecular beacons (MB-21) for detecting and intracellular imaging miRNA-21. The MLN-MB consists of three parts: aptamer, MLN, and MB-21. The aptamer (AS1411) could media MLN-MB enter live cells without additional transfection reagents. Once inside the cells, the intracellular miRNA-21 could hybridize the MB-21s, resulting in significantly enhanced fluorescence signals. The whole process was enzyme-free, autonomous, and continuous, which avoided the necessity of adding external fuel strands or enzymes. We demonstrated that the MLN-MB could be used to screen the miRNA-21 with a detection limit of 320 pM in a short time (10 min) and show high specificity toward miRNA-21 against other miRNAs. Moreover, the proposed MLN-MB could detect the miRNA-21 in complex matrixes stably. With its outstanding stability, dual recognition, and biocompatibility, MLN-MB is capable of delivering into living cells to identify specific cancer cells. Therefore, our sensing approach, with high sensitivity, specificity, and simplicity advantages, holds great potential for early cancer diagnosis.
Article numberARTN 123997
Publication classificationC1 Refereed article in a scholarly journal
Science & TechnologyPhysical SciencesChemistry, AnalyticalChemistryHybridization chain reaction (HCR)Multifunctional linear DNA nanostructure(MLN)microRNA imagingNuclease resistanceMESSENGER-RNA DETECTIONTUMOR-CELLSMICRORNAAMPLIFICATIONHYBRIDIZATIONSIGNALSMultifunctional linear DNA nanostructure (MLN)MicroRNAsDNACancerGeneticsBiotechnology4 Detection, screening and diagnosis4.1 Discovery and preclinical testing of markers and technologiesGeneric health relevanceChemical Sciences not elsewhere classifiedAnalytical Chemistry not elsewhere classified