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In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata
journal contribution
posted on 2003-06-01, 00:00 authored by M Maryani, M Morse, G Bradley, H Irving, David CahillDavid Cahill, Christoph GehringPlant natriuretic peptide immuno-analogues (irPNP) have previously been shown to affect a number of biological processes including stomatal guard cell movements, ion fluxes and osmoticum-dependent water transport. Tissue printing and immunofluorescent labelling techniques have been used here to study the tissue and cellular localization of irPNP in ivy (Hedera helix L.) and potato (Solanum tuberosum L.). Polyclonal antibodies active against human atrial natriuretic peptide (anti-hANP) and antibodies against irPNP from potato (anti-StPNP) were used for immunolabelling. Tissue prints revealed that immunoreactants are concentrated in vascular tissues of leaves, petioles and stems. Phloem-associated cells, xylem cells and parenchymatic xylem cells showed the strongest immunoreaction. Immunofluorescent microscopy with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG supported this finding and, furthermore, revealed strong labelling to stomatal guard cells and the adjacent apoplastic space as well. Biologically active immunoreactants were also detected in xylem exudates of a soft South African perennial forest sage (Plectranthus ciliatus E. Mey ex Benth.) thus strengthening the evidence for a systemic role of the protein. In summary, in situ cellular localization is consistent with physiological responses elicited by irPNPs reported previously and is indicative of a systemic role in plant homeostasis.
History
Journal
Journal of experimental botanyVolume
54Issue
387Pagination
1553 - 1564Publisher
Oxford University PressLocation
London, EnglandPublisher DOI
ISSN
0022-0957Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2003, Society for Experimental BiologyUsage metrics
Keywords
conductive tissueexpansinsHedera helix L.homeostasisimmuno-analoguesplant natriuretic peptidesPlectranthus ciliatus E. Mey ex Benth.Solanum tuberosum L.tissue printingScience & TechnologyLife Sciences & BiomedicinePlant SciencesPlectranthus ciliatus E. Mey ex BenthMESOPHYLL-CELLSROOT-GROWTHIMMUNOCYTOCHEMISTRYPOLYPEPTIDECYTOKININSMOLECULESEXPANSINDYNAMICSHORMONESPROTEINGeneticsPlant Biology
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