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suleria-invitroantiinflammatory-2017.pdf (1.85 MB)

In vitro anti-inflammatory activities of blacklip abalone (Haliotis rubra) in RAW 264.7 macrophages

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posted on 2017-07-04, 00:00 authored by Hafiz SuleriaHafiz Suleria, R Addepalli, P Masci, G Gobe, S A Osborne
© 2017 Informa UK Limited, trading as Taylor & Francis Group. Abalone (Haliotis sp) has been used as a traditional functional food for many years by different cultures believing that consumption provides health benefits. We investigated the anti-inflammatory activity from blacklip abalone visceral waste. An extract was prepared from the viscera using digestion with the food grade proteases papain and bromelain followed by anion exchange chromatography (AEC). The anti-inflammatory potential of the extract and AEC fractions were investigated in comparison to a plant-derived positive control (quercetin) through the inhibition of lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 monocytes. Significant anti-inflammatory activity was observed in response to the abalone extract and the unbound AEC material over a concentration (based on collagen) range of 12–96 µg/mL. Overall, results indicated that blacklip abalone extract has anti-inflammatory activity in vitro, warranting further investigation into the bioactive constituents that may be potential anti-inflammatory therapeutics.

History

Journal

Food and agricultural immunology

Volume

28

Issue

4

Pagination

711 - 724

Publisher

Taylor & Francis

Location

London, Eng.

ISSN

0954-0105

eISSN

1465-3443

Language

eng

Publication classification

C Journal article; C1.1 Refereed article in a scholarly journal

Copyright notice

2017, Informa UK Limited