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Inhibiting NOS blocks microvascular recruitment and blunts muscle glucose uptake in response to insulin.
journal contributionposted on 2003-07-01, 00:00 authored by Michelle KeskeMichelle Keske, E J Barrett, J R Lindner, M G Clark, S Rattigan
We examined the effects of inhibiting nitric oxide synthase with Nomega-nitro-l-arginine-methyl ester (l-NAME) on total hindlimb blood flow, muscle microvascular recruitment, and hindlimb glucose uptake during euglycemic hyperinsulinemia in vivo in the rat. We used two independent methods to measure microvascular perfusion. In one group of animals, microvascular recruitment was measured using the metabolism of exogenously infused 1-methylxanthine (1-MX), and in a second group contrast-enhanced ultrasound (CEU) was used. Limb glucose uptake was measured by arterial-venous concentration differences after 2 h of insulin infusion. Saline alone did not alter femoral artery flow, glucose uptake, or 1-MX metabolism. Insulin (10 mU.min-1.kg-1) significantly increased hindlimb total blood flow (0.69 +/- 0.02 to 1.22 +/- 0.11 ml/min, P < 0.05), glucose uptake (0.27 +/- 0.05 to 0.95 +/- 0.08 micromol/min, P < 0.05), 1-MX uptake (5.0 +/- 0.5 to 8.5 +/- 1.0 nmol/min, P < 0.05), and skeletal muscle microvascular volume measured by CEU (10.0 +/- 1.6 to 15.0 +/- 1.2 video intensity units, P < 0.05). Addition of l-NAME to insulin completely blocked the effect of insulin on both total limb flow and microvascular recruitment (measured using either 1-MX or CEU) and blunted glucose uptake by 40% (P < 0.05). We conclude that insulin specifically recruits flow to the microvasculture in skeletal muscle via a nitric oxide-dependent pathway and that this may be important to insulin's overall action to regulate glucose disposal.
JournalAmerican journal of physiology. Endocrinology and metabolism
PaginationE123 - E129
PublisherAmerican Physiological Society
Publication classificationC1.1 Refereed article in a scholarly journal
Copyright notice2003, American Physiological Society
CategoriesNo categories selected
AnimalsBlood GlucoseCapillariesEnzyme InhibitorsGlucoseHindlimbHypoglycemic AgentsInsulinMaleMuscle, SkeletalNG-Nitroarginine Methyl EsterNitric Oxide SynthaseNitric Oxide Synthase Type IIIRatsRats, Sprague-DawleyRegional Blood FlowUltrasonicsUltrasonographyXanthinesScience & TechnologyLife Sciences & BiomedicineEndocrinology & MetabolismPhysiologycapillary recruitmentnitric oxidemuscle blood flowNITRIC-OXIDE SYNTHASEHUMAN SKELETAL-MUSCLEMEDIATED CAPILLARY RECRUITMENTBLOOD-FLOWIN-VIVOHEMODYNAMIC ACTIONSRESISTANCEULTRASOUNDPERFUSIONQUANTIFICATION