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Insulin sensitivity of muscle capillary recruitment in vivo
journal contribution
posted on 2004-02-01, 00:00 authored by L Zhang, Michelle KeskeMichelle Keske, S M Richards, L H Clerk, S Rattigan, M G Clark, E J BarrettWe have reported that insulin exerts two vascular actions in muscle; it both increases blood flow and recruits capillaries. In parallel hyperinsulinemic-euglycemic clamp studies, we compared the insulin dose response of muscle microvascular recruitment and femoral blood flow as well as hindleg glucose uptake in fed, hooded Wistar and fasted Sprague-Dawley rats. Using insulin doses between 0 and 30 mU(-1). min(-1). kg(-1), we measured microvascular recruitment at 2 h by 1-methylxanthine (1-MX) metabolism or contrast-enhanced ultrasound (CEU), and muscle glucose uptake was measured by either arteriovenous differences or using 2-deoxyglucose. We also examined the time course for reversal of microvascular recruitment following cessation of a 3 mU. min(-1). kg(-1) insulin infusion. In both groups, whether measured by 1-MX metabolism or CEU, microvascular recruitment was fully activated by physiologic hyperinsulinemia and occurred at lower insulin concentrations than those that stimulated glucose uptake or hindleg total blood flow. The latter processes were insulin dose dependent throughout the entire dose range studied. Upon stopping the insulin infusion, increases in microvascular volume persisted for 15-30 min after insulin concentrations returned to basal levels. We conclude that the precapillary arterioles that regulate microvascular recruitment are more insulin sensitive than resistance arterioles that regulate total flow.
History
Journal
DiabetesVolume
53Issue
2Pagination
447 - 453Publisher
American Diabetes AssociationLocation
Arlington, Va.Publisher DOI
ISSN
0012-1797Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2004, American Diabetes AssociationUsage metrics
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No categories selectedKeywords
AnimalsBiological TransportBlood Flow VelocityBlood GlucoseCapillariesDeoxyglucoseGlucoseInsulinKineticsMaleMicrocirculationMuscle, SkeletalRatsRats, WistarRegional Blood FlowVascular ResistanceXanthinesScience & TechnologyLife Sciences & BiomedicineEndocrinology & MetabolismNITRIC-OXIDE RELEASERAT SKELETAL-MUSCLEGLUCOSE-UPTAKEBLOOD-FLOWHEMODYNAMIC ACTIONSHUMANSVASODILATIONINCREASESINFUSIONTISSUES
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