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Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis
journal contributionposted on 2011-01-01, 00:00 authored by Khashayar Khoshmanesh, J Akagi, Saeid Nahavandi, K Kalantar-Zadeh, Sara Baratchi, D Williams, J Cooper, D Wlodkowic
Development of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells.