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Interferon epsilon promotes HIV restriction at multiple steps of viral replication

journal contribution
posted on 2017-05-01, 00:00 authored by A Garcia-Minambres, S G Eid, N E Mangan, C Pade, S S Lim, A Y Matthews, N A de Weerd, P J Hertzog, Johnson Mak
Interferon epsilon (IFNɛ) is a type I IFN that is expressed constitutively in the female reproductive tract (FRT), and contributes to protection in models of sexually transmitted infections. Using multiple cell systems, including reporter cell lines and activated peripheral blood lymphocytes (PBLs), we show that recombinant IFNɛ impairs HIV infection at stage(s) post HIV entry and up to the translation of viral proteins. Consistent with this, IFNɛ upregulated a number of host cell restriction factors that block HIV at these stages of the replication cycle. The potency of IFNɛ induction of these HIV restriction factors was comparable to conventional type I IFNs, namely IFNα and IFNβ. IFNɛ also significantly reduced the infectivity of progeny virion particles likely by inducing expression of HIV restriction factors, such as IFITM3, which act at that stage of infection. Thus, our data demonstrate that human IFNɛ suppresses HIV replication at multiple stages of infection.

History

Journal

Immunology and cell biology

Volume

95

Issue

5

Pagination

478 - 483

Publisher

Nature Publishing Group

Location

London, United Kingdom

ISSN

0818-9641

eISSN

1440-1711

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2017, Australasian Society for Immunology