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Intermediate‐conductance calcium‐activated potassium channels in enteric neurones of the mouse: pharmacological, molecular and immunochemical evidence for their role in mediating the slow afterhyperpolarization

journal contribution
posted on 2024-08-23, 00:16 authored by Craig B Neylon, Kulmira Nurgali, Billie Hunne, Heather L Robbins, Stephen Moore, Mao Xiang Chen, John Furness
AbstractCalcium‐activated potassium channels are critically important in modulating neuronal cell excitability. One member of the family, the intermediate‐conductance potassium (IK) channel, is not thought to play a role in neurones because of its predominant expression in non‐excitable cells such as erythrocytes and lymphocytes, in smooth muscle tissues, and its lack of apparent expression in brain. In the present study, we demonstrate that IK channels are localized on specific neurones in the mouse enteric nervous system where they mediate the slow afterhyperpolarization following an action potential. IK channels were localized by immunohistochemistry on intrinsic primary afferent neurones, identified by their characteristic Dogiel type II morphology. The slow afterhyperpolarization recorded from these cells was abolished by the IK channel blocker clotrimazole. RT–PCR and western analysis of extracts from the colon revealed an IK channel transcript and protein identical to the IK channel expressed in other cell types. These results indicate that IK channels are expressed in neurones where they play an important role in modulating firing properties.

History

Journal

Journal of Neurochemistry

Volume

90

Pagination

1414-1422

Location

England

ISSN

0022-3042

eISSN

1471-4159

Language

en

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

6

Publisher

Wiley