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Investigating retention characteristics of a mixed-mode stationary phase and the enhancement of monolith selectivity for high-performance liquid chromatography

journal contribution
posted on 2014-08-01, 00:00 authored by Danielle Bassanese, A Soliven, Paul Stevenson, G Dennis, Neil BarnettNeil Barnett, R Shalliker, Xavier ConlanXavier Conlan
The synthesis and chromatographic behavior of an analytical size mixed-mode bonded silica monolith was investigated. The monolith was functionalized by an in situ modification process of a bare silica rod with chloro(3-cyanopropyl)dimethyl silane and chlorodimethyl propyl phenyl silane solutions. These ligands were selected in order to combine both resonance and nonresonance π-type bonding within a single separation environment. Selectivity studies were undertaken using n-alkyl benzenes and polycyclic aromatic hydrocarbons in aqueous methanol and acetonitrile mobile phases to assess the methylene and aromatic selectivities of the column. The results fit with the linear solvent strength theory suggesting excellent selectivity of the column was achieved. Comparison studies were performed on monolithic columns that were functionalized separately with cyano and phenyl ligands, suggesting highly conjugated molecules were able to successfully exploit both of the π-type selectivities afforded by the two different ligands on the mixed-mode column.

History

Journal

Journal of separation science

Volume

37

Issue

15

Pagination

1937 - 1943

Publisher

Wiley

Location

Weinheim, Germany

ISSN

1615-9306

eISSN

1615-9314

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2014, Wiley