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Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhea in Canada
journal contribution
posted on 2014-10-01, 00:00 authored by J Pasick, Y Berhane, D Ojkic, G Maxie, C Embury-Hyatt, K Swekla, K Handel, J Fairles, Soren AlexandersenSoren AlexandersenIn January 2014, approximately 9 months following the initial detection of porcine epidemic diarrhea (PED) in the USA, the first case of PED was confirmed in a swine herd in south-western Ontario. A follow-up epidemiological investigation carried out on the initial and 10 subsequent Ontario PED cases pointed to feed as a common risk factor. As a result, several lots of feed and spray-dried porcine plasma (SDPP) used as a feed supplement were tested for the presence of PEDV genome by real-time RT-PCR assay. Several of these tested positive, supporting the notion that contaminated feed may have been responsible for the introduction of PEDV into Canada. These findings led us to conduct a bioassay experiment in which three PEDV-positive SDPP samples (from a single lot) and two PEDV-positive feed samples supplemented with this SDPP were used to orally inoculate 3-week-old piglets. Although the feed-inoculated piglets did not show any significant excretion of PEDV, the SDPP-inoculated piglets shed PEDV at a relatively high level for ≥9 days. Despite the fact that the tested PEDV genome positive feed did not result in obvious piglet infection in our bioassay experiment, contaminated feed cannot be ruled out as a likely source of this introduction in the field where many other variables may play a contributing role.
History
Journal
Transboundary and emerging diseasesVolume
61Issue
5Pagination
397 - 410Publisher
WileyLocation
London, Eng.Publisher DOI
Link to full text
ISSN
1865-1682eISSN
1865-1682Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2014, WileyUsage metrics
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No categories selectedKeywords
porcine epidemic diarrheaspray-dried porcine plasmatransmissionAnimal FeedAnimalsCanadaCoronavirus InfectionsDiarrheaDisease OutbreaksFood ContaminationMolecular Sequence DataPorcine epidemic diarrhea virusSwineSwine DiseasesScience & TechnologyLife Sciences & BiomedicineInfectious DiseasesVeterinary SciencesUNITED-STATESEXPERIMENTAL-INFECTIONCIRCOVIRUS TYPE-2VIRUSCORONAVIRUSPIGSPLASMA
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