File(s) not publicly available
Isolating conifer DNA: a superior polysaccharide elimination method
journal contributionposted on 2003-03-01, 00:00 authored by Tamsyn CrowleyTamsyn Crowley, Morley Muralitharan, T Stevenson
Genomic DNA was isolated from frozen needles of maturePinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility.