Deakin University
Browse

File(s) under permanent embargo

Isolation and characterisation of polymorphic microsatellite loci for Noisy Miners Manorina melanocephala, with successful cross-amplification in Bell Miners M. melanophrys

journal contribution
posted on 2013-03-01, 00:00 authored by A Kopps, P McDonald, Lee Rollins
We characterized 15 polymorphic microsatellite loci identified from a Noisy Miner (Manorina melanocephala) blood sample using 454 whole genome shotgun sequencing. Levels of polymorphism were assessed using 15 Noisy Miners. The average number of alleles per locus was 5.1. These loci were then cross-amplified to assess their suitability in a single population of Bell Miners (M. melanophrys). Given the landscape level impact that these species are having on the health of vegetation and biodiversity of a range of vertebrates throughout much of south-eastern Australia, these primers will help identify colony dispersal patterns and thus aid in modeling predictions of miner presence and tenure length in threatened ecosystems.

History

Journal

Conservation genetics resources

Volume

5

Issue

1

Pagination

39 - 41

Publisher

Springer Netherlands

Location

Dordrecht, The Netherlands

ISSN

1877-7252

eISSN

1877-7260

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2012, Springer Science+Business Media B.V.