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Isolation of a human ceruloplasmin cDNA clone that includes the N-terminal leader sequence
journal contributionposted on 2023-02-07, 04:11 authored by Julian MercerJulian Mercer, A Grimes
A number of cDNA clones encoding human ceruloplasmin were identified using two mixed oligonucleotide probes. One of these clones was shown by DNA sequence analysis to span from the complete N-terminal leader sequence to 114 amino acids short of the C-terminus. The leader sequence consists of 19 primarily hydrophobic amino acids. Northern blot analysis of RNA from human liver showed two species of ceruloplasmin mRNA; a minor species of 3600 nucleotides and a major one of 4400 nucleotides. © 1986.