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Kinetic optimisation of open-tubular liquid-chromatography capillaries coated with thick porous layers for increased loadability
journal contribution
posted on 2011-11-18, 00:00 authored by Tim J Causon, Robert ShellieRobert Shellie, Emily F Hilder, Gert Desmet, Sebastiaan EeltinkThe kinetic optimisation of open-tubular liquid chromatography (OTLC) columns has been revisited by taking the thick-film effects for porous coatings on retention, column resistance, band broadening and mass loadability into account. Considering the most advantageous case (i.e. where the retentive layer allows for the same high internal diffusion coefficient as conventional porous particles), calculations show the need for the development of coating procedures leading to porous films filling up approximately 50-70% of the total column diameter. Furthermore, to achieve optimum kinetic performance for separations of small molecules with total analysis times of less than 8h (k'=9), total column diameters should be less than 6 μm with lengths typically greater than 0.8m for N values of 125,000-500,000 at a pressure of 400 bar. The use of elevated temperature LC (90°C) is also shown to increase the allowable total column diameter to up to 9 μm for a larger range of N values (100,000-880,000).
History
Journal
Journal of chromatography AVolume
1218Issue
46Pagination
8388 - 8393Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
eISSN
1873-3778Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2011, Elsevier B.V.Usage metrics
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No categories selectedKeywords
Chromatography, LiquidKineticsModels, TheoreticalParticle SizePorosityPressureTemperaturePLOT columnsCoatingKinetic plotsScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryPROTEOMIC ANALYSISCONVENTIONAL COLUMNSHIGHLY EFFICIENTMASS LOADABILITYSILICA LAYERSANALYSIS TIMEPLOT METHODPERFORMANCEPARTICLES
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