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Kinetic performance appraisal of poly(styrene-co-divinylbenzene) monolithic high-performance liquid chromatography columns for biomolecule analysis
journal contributionposted on 2010-06-11, 00:00 authored by Tim J Causon, Robert ShellieRobert Shellie, Emily F Hilder
A broad appraisal of the kinetic performance of organic polymeric monolithic columns is reported using commercially available poly(styrene-co-divinylbenzene) monolithic columns (Dionex ProSwift RP-1S). Analysis of a protein digest sample at elevated temperatures (> or =80 degrees C) indicated no apparent analyte degradation using an inert polymeric stationary phase. Comparison between low molecular weight solute and peptide separations highlighted the markedly different mass transport processes observed on macroporous monolithic beds and an improved C term at elevated temperature in both instances. The current usefulness of this column format for biomolecule analysis was further studied via employment of a kinetic performance characterisation for the first time to provide direction for column development servicing this application.
JournalJournal of chromatography A
Pagination3765 - 3769
LocationAmsterdam, The Netherlands
Publication classificationC1.1 Refereed article in a scholarly journal
Copyright notice2010, Elsevier B.V.
CategoriesNo categories selected
Angiotensin IIChromatography, High Pressure LiquidCytochromes cHot TemperatureKineticsPeptide FragmentsPolystyrenesTrypsinScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryPolymer monolithsKinetic performanceBioseparationsPeptidesHigh temperature liquid chromatographyREVERSED-PHASESTATIONARY-PHASECAPILLARY COLUMNSPLOT METHODELEVATED-TEMPERATURESIZE DISTRIBUTIONPARTICLE-SIZEPEAK-CAPACITYMOBILE-PHASELC SUPPORTS