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Kinetic performance optimisation for liquid chromatography: principles and practice
journal contribution
posted on 2011-04-01, 00:00 authored by Tim J Causon, Ken Broeckhoven, Emily F Hilder, Robert ShellieRobert Shellie, Gert Desmet, Sebastiaan EeltinkThis HPLC tutorial focuses on the preparation and use of kinetic plots to characterise the performance in isocratic and gradient LC. This graphical approach allows the selection of columns (i.e. optimum particle size and column length) and LC conditions (operating pressure and temperature) to generate a specific number of plates or peak capacity in the shortest possible analysis time. Instrument aspects including the influence of extra-column effects (maximum allowable system volume) and thermal operating conditions (oven type) on performance are discussed. In addition, the performance characteristics of porous-shell particle-packed columns and monolithic stationary phases are presented and the potential of future column designs is discussed.
History
Journal
Journal of separation scienceVolume
34Issue
8Pagination
877 - 887Publisher
John Wiley & SonsLocation
Chichester, Eng.Publisher DOI
eISSN
1615-9314Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2011, WILEY‐VCH Verlag GmbH & Co. KGaA, WeinheimUsage metrics
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No categories selectedKeywords
High temperatureKinetic plotMonolithPoppe plotUltra high‐pressure liquid chromatographyScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryUltra high-pressure liquid chromatographyULTRA-HIGH-PRESSUREMONOLITHIC CAPILLARY COLUMNSTHERMAL CONDITIONSMASS-SPECTROMETRYGRADIENT-ELUTIONSEPARATION MEDIAPOROUS PARTICLESPEAK-CAPACITYBORE COLUMNSPLOT METHOD
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