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LC–MS and GC–MS metabolite profiling of nickel(II) complexes in the latex of the nickel-hyperaccumulating tree Sebertia acuminata and identification of methylated aldaric acid as a new nickel(II) ligand
journal contribution
posted on 2008-01-01, 00:00 authored by Damien CallahanDamien Callahan, U Roessner, V Dumontet, N Perrier, A G Wedd, R A J O’Hair, A J M Baker, S D KolevTargeted liquid chromatography–mass spectrometry (LC–MS) technology using size exclusion chromatography and metabolite profiling based on gas chromatography–mass spectrometry (GC–MS) were used to study the nickel-rich latex of the hyperaccumulating tree Sebertia acuminata. More than 120 compounds were detected, 57 of these were subsequently identified. A methylated aldaric acid (2,4,5-trihydroxy-3-methoxy-1,6-hexan-dioic acid) was identified for the first time in biological extracts and its structure was confirmed by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. After citric acid, it appears to be one of the most abundant small organic molecules present in the latex studied. Nickel(II) complexes of stoichiometry NiII:acid = 1:2 were detected for these two acids as well as for malic, itaconic, erythronic, galacturonic, tartaric, aconitic and saccharic acids. These results provide further evidence that organic acids may play an important role in the transport and possibly in the storage of metal ions in hyperaccumulating plants.
History
Journal
PhytochemistryVolume
69Issue
1Pagination
240 - 251Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0031-9422Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2008, ElsevierUsage metrics
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