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Lack of ceruloplasmin expression alters aspects of copper transport to the fetus and newborn, as determined in mice
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posted on 2012-04-01, 00:00 authored by Y L Chu, E Sauble, A Cabrera, A Roth, Leigh AcklandLeigh Ackland, Julian MercerJulian Mercer, M LinderCopper transport and accumulation were studied in virgin and lactating C57BL/6 mice, with and without expression of ceruloplasmin (Cp), to assess the importance of Cp to these processes. One hour after i.p. injection of tracer 64Cu, liver and kidney accounted for 80% of the radioactivity, and mammary gland 1%, while in lactating Cp+/+ mice 2–4 days post partum, uptake by mammary gland was 9-fold higher and that of liver and other organs was decreased, with 64Cu rapidly appearing in milk. Parallel studies in Cp−/− mice (siblings from same colony) gave virtually identical results. However, their milk contained less 64Cu, and actual copper contents determined by furnace atomic absorption were less than half those for milk from normal dams. Liver copper concentrations of pups born to Cp−/− dams also were half those of pups from wild type dams. Copper in pup brains was unaffected; but iron concentrations were reduced. We conclude that absence of Cp, while not affecting entry of exchangeable copper from the blood into the mammary gland, does have a significant effect on the availability of this metal to the newborn through the milk and in the form of stores accumulating in gestation.
History
Journal
BioMetalsVolume
25Issue
2Pagination
373 - 382Publisher
Springer NetherlandsLocation
Dordrecht, The NetherlandsISSN
0966-0844eISSN
1572-8773Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2011, Springer Science+Business MediaUsage metrics
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