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Landscape of DNA methylation on the marsupial x

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Version 2 2024-06-04, 13:35
Version 1 2018-07-10, 11:09
journal contribution
posted on 2024-06-04, 13:35 authored by SA Waters, AM Livernois, H Patel, D O Meally, Jeffrey CraigJeffrey Craig, JA Marshall Graves, CM Suter, PD Waters
© The Author 2017. DNA methylation plays a key role in maintaining transcriptional silence on the inactive X chromosome of eutherian mammals. Beyond eutherians, there are limited genome wide data on DNA methylation from other vertebrates. Previous studies of X borne genes in various marsupial models revealed no differential DNA methylation of promoters between the sexes, leading to the conclusion that CpG methylation plays no role in marsupial X-inactivation. Using reduced representation bisulfite sequencing, we generated male and female CpG methylation profiles in four representative vertebrates (mouse, gray short-tailed opossum, platypus, and chicken). A variety of DNA methylation patterns were observed. Platypus and chicken displayed no large-scale differential DNA methylation between the sexes on the autosomes or the sex chromosomes. As expected, a metagene analysis revealed hypermethylation at transcription start sites (TSS) of genes subject to X-inactivation in female mice. This contrasted with the opossum, in which metagene analysis did not detect differential DNA methylation between the sexes at TSSs of genes subject to X-inactivation. However, regions flanking TSSs of these genes were hypomethylated. Our data are the first to demonstrate that, for genes subject to X-inactivation in both eutherian and marsupial mammals, there is a consistent difference between DNA methylation levels at TSSs and immediate flanking regions, which we propose has a silencing effect in both groups.

History

Journal

Molecular biology and evolution

Volume

35

Pagination

431-439

Location

Oxford, Eng.

Open access

  • Yes

ISSN

0737-4038

eISSN

1537-1719

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2017, The Authors

Issue

2

Publisher

Oxford University Press

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