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Late-gestation maternal dietary methyl donor and cofactor supplementation in sheep partially reverses protection against allergic sensitization by IUGR
journal contribution
posted on 2018-01-01, 00:00 authored by Amy L Wooldridge, Robert J Bischof, Hong Liu, Gary K Heinemann, Damien S Hunter, Lynne C Giles, Rebecca A Simmons, Yu-Chin Lien, Wenyun Lu, Joshua D Rabinowitz, Karen L Kind, Julie OwensJulie Owens, Vicki L Clifton, Kathryn L GatfordPerinatal exposures are associated with altered risks of childhood allergy. Human studies and our previous work suggest that restricted growth in utero (IUGR) is protective against allergic disease. The mechanisms are not clearly defined, but reduced fetal abundance and altered metabolism of methyl donors are hypothesized as possible underlying mechanisms. Therefore, we examined whether late-gestation maternal dietary methyl donor and cofactor supplementation of the placentally restricted (PR) sheep pregnancy would reverse allergic protection in progeny. Allergic outcomes were compared between progeny from control pregnancies (CON; n = 49), from PR pregnancies without intervention (PR; n = 28), and from PR pregnancies where the dam was fed a methyl donor plus cofactor supplement from day 120 of pregnancy until delivery (PR + Methyl; n = 25). Both PR and PR + Methyl progeny were smaller than CON; supplementation did not alter birth size. PR was protective against cutaneous hypersensitivity responses to ovalbumin (OVA; P < 0.01 in singletons). Cutaneous hypersensitivity responses to OVA in PR + Methyl progeny were intermediate to and not different from the responses of CON and PR sheep. Cutaneous hypersensitivity responses to house dust mites did not differ between treatments. In singleton progeny, upper dermal mast cell density was greater in PR + Methyl than in PR or CON (each P < 0.05). The differences in the cutaneous allergic response were not explained by treatment effects on circulating immune cells or antibodies. Our results suggest that mechanisms underlying in utero programming of allergic susceptibility by IUGR and methyl donor availability may differ and imply that late-gestation methyl donor supplementation may increase allergy risk.
History
Journal
American journal of physiology: regulatory, integrative and comparative physiologyVolume
314Issue
1Pagination
R22 - R33Publisher
American Physiological SocietyLocation
Bethesda, Md.Publisher DOI
Link to full text
eISSN
1522-1490Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2017 the American Physiological SocietyUsage metrics
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animal modelsfetal growthfolic acidintrauterine growth restrictionmast cellsmethyl donorsAnimalsCobaltDNA MethylationDermatitisDietary SupplementsDisease Models, AnimalFemaleFetal Growth RetardationGestational AgeHypersensitivityImmunoglobulin EMethionineOvalbuminPlacentaPregnancyPrenatal Exposure Delayed EffectsPyroglyphidaeSheep, DomesticSkinSulfurScience & TechnologyLife Sciences & BiomedicinePhysiologyFETAL-GROWTHFOLIC-ACIDHOMOCYSTEINE CONCENTRATIONSPLACENTAL RESTRICTIONMETABOLOMIC ANALYSISATOPIC-DERMATITISAIRWAY DISEASEFOLATE STATUSCELL NUMBERS
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