Leg muscle pH following sprint running

In an effort to compare the disturbances in leg muscle pH during sprint running, muscle biopsies were obtained from the gastrocnemius and vastus lateralis muscles of six healthy men (three endurance-trained and three nonendurance-trained) before and after a treadmill sprint run (TSR) to fatigue (54-105 s) at roughly 125% of their aerobic capacities. Following the TSR, repeated blood samples were taken from a hand vein and later analyzed for pH, PCO2, and lactic acid (HLa). The muscle specimens were analyzed in duplicate for pH and HLa. Resting-muscle pH was 7.03 +/- 0.02 (means +/- SE) and 7.04 +/- 0.01 for the gastrocnemius and vastus lateralis muscles, respectively. At the termination of the TSR, the pH in these muscles was 6.88 +/- 0.05 and 6.86 +/- 0.03, respectively. After a 400-m timed run on the track, the pH in the gastrocnemius of four of the subjects averaged 6.63 +/- 0.03, while blood pH and HLa were 7.10 +/- 0.03 and 12.3 mM, respectively. Although no differences in pH and HLa were observed between the vastus lateralis and gastrocnemius muscles at the end of the treadmill trial, it is speculated that the lesser disturbance in acid-base balance seen in endurance performers may have been due to a lesser production of metabolites in their running musculature when compared to nonendurance performers.