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Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography

journal contribution
posted on 2017-01-01, 00:00 authored by M E Creese, M J Creese, J P Foley, H J Cortes, E F Hilder, Robert Shellie, M C Breadmore
© 2016 American Chemical Society. Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6-14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30-55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.

History

Journal

Analytical Chemistry

Volume

89

Issue

2

Pagination

1123 - 1130

Publisher

ASC Publications

Location

Washington, D.C.

ISSN

0003-2700

eISSN

1520-6882

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2016, American Chemical Society

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