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Making a hydrophoretic focuser tunable using a diaphragm

journal contribution
posted on 2023-02-07, 04:31 authored by S Yan, Jun ZhangJun Zhang, H Chen, G Alici, H Du, Y Zhu, W Li
Microfluidic diagnostic devices often require handling particles or cells with different sizes. In this investigation, a tunable hydrophoretic device was developed which consists of a polydimethylsiloxane (PDMS) slab with hydrophoretic channel, a PDMS diaphragm with pressure channel, and a glass slide. The height of the hydrophoretic channel can be tuned simply and reliably by deforming the elastomeric diaphragm with pressure applied on the pressure channel. This operation allows the device to have a large operating range where different particles and complex biological samples can be processed. The focusing performance of this device was tested using blood cells that varied in shape and size. The hydrophoretic channel had a large cross section which enabled a throughput capability for cell focusing of ~15 000 cells s-1, which was more than the conventional hydrophoretic focusing and dielectrophoresis (DEP)-active hydrophoretic methods. This tunable hydrophoretic focuser can potentially be integrated into advanced lab-on-a-chip bioanalysis devices.

History

Journal

Biomicrofluidics

Volume

8

Article number

ARTN 064115

Location

United States

ISSN

1932-1058

eISSN

1932-1058

Language

English

Issue

6

Publisher

AMER INST PHYSICS