craig-maternalvitamind-2012.pdf (422.9 kB)
Maternal vitamin D predominates over genetic factors in determining neonatal circulating vitamin D concentrations
journal contribution
posted on 2012-07-01, 00:00 authored by Boris Novakovic, John C Galati, Anna Chen, Ruth Morley, Jeffrey CraigJeffrey Craig, Richard SafferyBACKGROUND: There are multiple potential regulators of neonatal vitamin D status of environmental, genetic, and epigenetic origins. The relation between these factors and circulating neonatal vitamin D has yet to be fully characterized. OBJECTIVE: The aim of this study was to examine the relative contribution of genetic factors, maternal circulating 25-hydroxyvitamin D [25(OH)D] concentrations, and the placental methylation level of the gene that encodes the primary catabolic enzyme of active vitamin D [25(OH)D-24-hydroxylase encoded by CYP24A1] to neonatal 25(OH)D concentrations. DESIGN: We used the classical twin study design to determine the genetic contribution to neonatal 25(OH)D. A total of 86 twin pairs (32 monozygotic and 54 dizygotic twin pairs) were included in this study. Serum 25(OH)D was measured by using a 25(OH)D kit. CYP24A1 promoter DNA methylation was measured by means of matrix-assisted laser desorption time-of-flight mass spectrometry. RESULTS: Maternal and neonatal 25(OH)D showed a strong association (R² = 0.19). Monozygotic and dizygotic within-pair serum 25(OH)D correlations were similar (R² = 0.71 and 0.67, respectively), which suggested no genetic effect. Placental CYP24A1 methylation did not show an association with maternal or neonatal 25(OH)D concentrations. CONCLUSIONS: Our results suggest that maternal circulating 25(OH)D is the most significant regulator of neonatal circulating 25(OH)D concentrations, with underlying genetic factors playing a limited role. The placental methylation of the CYP24A1 promoter appears subject to a genetic influence, although no evidence of a relation between the methylation level of this gene and circulating maternal or neonatal 25(OH)D was apparent.
History
Journal
American journal of clinical nutritionVolume
96Issue
1Pagination
188 - 195Publisher
Oxford University PressLocation
Oxford, Eng.Publisher DOI
Link to full text
ISSN
0002-9165eISSN
1938-3207Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2012, American Society for NutritionUsage metrics
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25-Hydroxyvitamin D 2CalcifediolDNA MethylationFemaleFetal BloodHumansInfant, NewbornMaleMaternal Nutritional Physiological PhenomenaPlacentaPregnancyPregnancy ComplicationsPromoter Regions, GeneticSteroid HydroxylasesTwins, DizygoticTwins, MonozygoticVictoriaVitamin D DeficiencyVitamin D3 24-HydroxylaseScience & TechnologyLife Sciences & BiomedicineNutrition & DieteticsPROMOTER METHYLATIONSERUM 1,25-DIHYDROXYVITAMIN-DD 24-HYDROXYLASE\/CYP24A125-HYDROXYVITAMIN DHUMAN PLACENTAINFANT GROWTHZYGOSITYPATTERNSREVEALS
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