Deakin University
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MeCP2 interacts with chromosomal microRNAs in brain

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posted on 2017-12-02, 00:00 authored by A W Khan, Mark Ziemann, H Rafehi, S Maxwell, G D Ciccotosto, A El-Osta
© 2018 Taylor & Francis Group, LLC. Although methyl CpG binding domain protein-2 (MeCP2) is commonly understood to function as a silencing factor at methylated DNA sequences, recent studies also show that MeCP2 can bind unmethylated sequences and coordinate gene activation. MeCP2 displays broad binding patterns throughout the genome, with high expression levels similar to histone H1 in neurons. Despite its significant presence in the brain, only subtle gene expression changes occur in the absence of MeCP2. This may reflect a more complex regulatory mechanism of MeCP2 to complement chromatin binding. Using an RNA immunoprecipitation of native chromatin technique, we identify MeCP2 interacting microRNAs in mouse primary cortical neurons. In addition, comparison with mRNA sequencing data from Mecp2-null mice suggests that differentially expressed genes may indeed be targeted by MeCP2-interacting microRNAs. These findings highlight the MeCP2 interaction with microRNAs that may modulate its binding with chromatin and regulate gene expression.

History

Journal

Epigenetics

Volume

12

Issue

12

Pagination

1028 - 1037

Publisher

Taylor & Francis

Location

Abingdon, Eng.

ISSN

1559-2294

eISSN

1559-2308

Language

eng

Publication classification

C Journal article; C1.1 Refereed article in a scholarly journal

Copyright notice

2018, Taylor & Francis Group