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Measurement of lactose crystallinity using Raman spectroscopy

journal contribution
posted on 2005-06-01, 00:00 authored by Bridget Murphy, S Prescott, I Larson
Raman spectroscopy (RS) was used to determine the crystallinity of lactose (a commonly used carrier in dry powder inhaler (DPI) formulations). Samples of α-lactose monohydrate and amorphous lactose were prepared using ethanol precipitation and lyophilisation respectively. The anomeric forms were confirmed using DSC at a rate of 10 °C/min and heated to 250 °C. The Raman spectra of both α-lactose monohydrate and amorphous lactose were obtained. Distinguishable differences were seen between the two spectra including peak areas and intensities. Depolarisation ratios (ρ) of each form were then determined to identify the crystallinity of the lactose carrier samples. At the prominent Raman bands 865 and 1082 cm−1, significant differences in ρ values were observed for crystalline (0.80 ± 0.07, 0.89 ± 0.06 respectively) and amorphous samples (0.44 ± 0.07, 0.51 ± 0.10).

History

Journal

Journal of pharmaceutical and biomedical analysis

Volume

38

Issue

1

Pagination

186 - 190

Publisher

Elsevier BV

Location

Amsterdam, Netherlands

ISSN

0731-7085

eISSN

1873-264X

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2004, Elsevier B.V.

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