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Membrane assisted and temperature controlled on-line evaporative concentration for microfluidics
journal contribution
posted on 2017-02-24, 00:00 authored by E Fornells, B Barnett, M Bailey, Robert ShellieRobert Shellie, E F Hilder, M C BreadmoreA membrane evaporation concentrator for continuous flow conditions is introduced. The membrane evaporation concentrator provides nearly 30-fold concentration in less than 60min whilst maintaining solute integrity under different sub-ambient pressure conditions and mild temperatures. To better understand the performance of the concentrator, a theoretical model was developed using caffeine as a model analyte, and used to predict the concentration performance of three target analytes at different conditions. An exponential relationship exists between temperature and concentration factor. By using the model it was determined that a 10-fold concentration (±0.5) can be performed at 56.72±0.07°C and at a flow rate of 10μLmin-1. Altogether, the model provides a better understanding of the process and ease of application in a wide variety of analytical methods. This work demonstrates that it is possible to obtain high concentrations with a continuously flowing fluid when temperature is precisely controlled and in times that are reasonable compared to existing evaporation concentration procedures.
History
Journal
Journal of chromatography aVolume
1486Pagination
110 - 116Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0021-9673eISSN
1873-3778Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2016, Elsevier B.V.Usage metrics
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No categories selectedKeywords
EvaporationPreconcentrationCaffeineMembranes, ArtificialMicrofluidicsPressureTemperatureVolatilizationScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistrySOLID-PHASE EXTRACTIONSAMPLE PRECONCENTRATIONDEVICESELECTROPHORESISDISTILLATIONCHIPCHROMATOGRAPHYSEPARATIONCAPILLARYPROTEINS
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