singh-metalionloaded-2017.pdf (1.19 MB)
Download fileMetal ion-loaded nanofibre matrices for calcification inhibition in polyurethane implants
journal contribution
posted on 2017-09-01, 00:00 authored by Charanpreet Singh, Xungai WangPathologic calcification leads to structural deterioration of implant materials via stiffening, stress cracking, and other structural disintegration mechanisms, and the effect can be critical for implants intended for long-term or permanent implantation. This study demonstrates the potential of using specific metal ions (MI)s for inhibiting pathological calcification in polyurethane (PU) implants. The hypothesis of using MIs as anti-calcification agents was based on the natural calcium-antagonist role of Mg(2+) ions in human body, and the anti-calcification effect of Fe(3+) ions in bio-prosthetic heart valves has previously been confirmed. In vitro calcification results indicated that a protective covering mesh of MI-doped PU can prevent calcification by preventing hydroxyapatite crystal growth. However, microstructure and mechanical characterisation revealed oxidative degradation effects from Fe(3+) ions on the mechanical properties of the PU matrix. Therefore, from both a mechanical and anti-calcification effects point of view, Mg(2+) ions are more promising candidates than Fe(3+) ions. The in vitro MI release experiments demonstrated that PU microphase separation and the structural design of PU-MI matrices were important determinants of release kinetics. Increased phase separation in doped PU assisted in consistent long-term release of dissolved MIs from both hard and soft segments of the PU. The use of a composite-sandwich mesh design prevented an initial burst release which improved the late (>20 days) release rate of MIs from the matrix.
History
Journal
Journal of functional biomaterialsVolume
8Issue
3Article number
22Pagination
1 - 16Publisher
MDPI AGLocation
Basel, SwitzerlandPublisher DOI
eISSN
2079-4983Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2017, The AuthorsUsage metrics
Categories
No categories selectedKeywords
calcificationmagnesiummetal ionVon Kossa methodAlizarin red S staininganti-calcificationnanofibre matrixhydroxyapatiteScience & TechnologyTechnologyEngineering, BiomedicalMaterials Science, BiomaterialsEngineeringMaterials ScienceVASCULAR CALCIFICATIONIN-VITROSERUM MAGNESIUMARTERIAL CALCIFICATIONINVITRO MODELCALCIUMRELEASEDELIVERYSUPPLEMENTATIONMECHANISMS